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Biomolecule-nanoparticle interactions: Elucidation of the thermodynamics by isothermal titration calorimetry

TitleBiomolecule-nanoparticle interactions: Elucidation of the thermodynamics by isothermal titration calorimetry
Publication TypeJournal Article
Year of Publication2016
AuthorsHuang R, Lau BLT
JournalBiochim Biophys Acta – Gen Subjects
Volume1860
Issue5
Start Page945
Pagination945-956
Date Published05/2016
KeywordsBinding affinity, Heat exchange, Isothermal titration calorimetry, nanoparticles, protein corona
Abstract

Nanomaterials (NMs) are often exposed to a broad range of biomolecules of different abundances. Biomolecule sorption driven by various interfacial forces determines the surface structure and composition of NMs, subsequently governs their functionality and the reactivity of the adsorbed biomolecules. Isothermal titration calorimetry (ITC) is a nondestructive technique that quantifies thermodynamic parameters through in-situ measurement of the heat absorption or release associated with an interaction.

DOI10.1016/j.bbagen.2016.01.027