|Title||Biomolecule-nanoparticle interactions: Elucidation of the thermodynamics by isothermal titration calorimetry|
|Publication Type||Journal Article|
|Year of Publication||2016|
|Authors||Huang R, Lau BLT|
|Journal||Biochim Biophys Acta – Gen Subjects|
|Keywords||Binding affinity, Heat exchange, Isothermal titration calorimetry, nanoparticles, protein corona|
Nanomaterials (NMs) are often exposed to a broad range of biomolecules of different abundances. Biomolecule sorption driven by various interfacial forces determines the surface structure and composition of NMs, subsequently governs their functionality and the reactivity of the adsorbed biomolecules. Isothermal titration calorimetry (ITC) is a nondestructive technique that quantifies thermodynamic parameters through in-situ measurement of the heat absorption or release associated with an interaction.